ABSTRACT
The microdilution technique, using a microplate photometer, to determine the minimal inhibitory concentration (MIC) for a natural product was compared to the serial tube dilution method. The MIC obtained for Paepalantine against S. aureus was the same by the two methods, showing an antimicrobial effect similar to chloramphenicol.
Subject(s)
Ampicillin , In Vitro Techniques , Drug Resistance, Microbial , Enzyme-Linked Immunosorbent Assay , MethodsABSTRACT
Microbiological routine exams of endocervix and vaginal specimens of 22 women with clinical history of recent spontaneous abortion or premature rupture of membranes were accomplished. "Chlamydia thachomatis", "Streptococcus pyogenes", "Streptococcus agalactie", "Candida" sp and "Gardnerela vaginalis" were recovered from 54.5 (per cent) (12) of the women. "Ureoplasma urealyticum" was frequently isolated (45.5 (per cent)) but 5 out of 22 qualitative investigation on genital microflora in pregnant women, since it is likely to influence onm pregnancy outcome.
Subject(s)
Pregnancy , Humans , Female , Adult , Bacteria, Aerobic/isolation & purification , Vagina/microbiology , Fetal Membranes, Premature Rupture/microbiology , Cervix Uteri/microbiology , Abortion, Spontaneous/microbiology , Streptococcus agalactiae/isolation & purification , Streptococcus pyogenes/isolation & purification , Candida/isolation & purification , Chlamydia trachomatis/isolation & purification , Gardnerella vaginalis/isolation & purificationABSTRACT
Resumo: Cultura quantitativa para Ureaplasma urealyticum foi realizada a partir de urina colhida, após antissepsia, de primeiro jato e jato médio com o objetivo de detectar a presença desse molicute no trato urinário. Os resultados, expressos em CCu (color changing units), mostraram que 14 (63 por cento) dos pacientes com cultura positiva albergavam o microrganismo em quantidades iguais ou superiores a 10 (elevado a sete) CCU/ml nas porçöes de urina obtidas de primeiro jato e jato médio. Näo foram observadas alteraçöes químicas ou microscópicaas (proteinas, leucócitos) nos materiais examinados. U. urealyticum em amostra de urina colhida de jato médio, mesmo em quantidade considerável, näo elucida as patogenicidade desse microrganismo (au)